HSSC Lab Technician Syllabus 2026 covers Group 49 recruitment for Lab Technician posts under the Haryana Staff Selection Commission. This HSSC Lab Technician Syllabus guide breaks down the exam pattern and the full subject-wise topic list, straight from the official syllabus published at hssc.gov.in, so you know exactly what to study before you open a single PDF.
HSSC Lab Technician Syllabus Exam Pattern
Group 49 recruitment follows the standard two-stage HSSC process. Stage one is the Common Eligibility Test (CET) — 100 questions, 100 marks, roughly 90–105 minutes, covering general awareness, reasoning, Hindi, English and quantitative aptitude. Clearing CET plus the socio-economic criteria stage qualifies you for the group-specific written exam, which is where the HSSC Lab Technician Syllabus below actually applies.
The HSSC Lab Technician Syllabus for the written exam splits into weighted parts:
- General awareness, Reasoning, Mathematics, Science, History including Haryana related history, current affairs, literature, Geography, Civics, Environment, Culture etc — 20% weightage
- Computer terminology, Fundamentals, word software, excel software, Power point, internet, web browsing, Communication, emails, downloading and uploading data on websites etc — 10% weightage
- Subject related syllabus — 70% weightage
HSSC notes on the official document that this weightage is tentative and may vary, so treat it as a study-time guide rather than a locked question count.
HSSC Lab Technician Syllabus 2026 (Subject-Wise)
The subject-specific block of the HSSC Lab Technician Syllabus spans the following areas. Here is the complete breakdown.
Electromagnetic waves: Basic Idea of displacement current, Electromagnetic waves, Sources, and Characteristics of EMW, Transverse Nature of EMW (qualitative Ideas only) Electromagnetic Spectrum, (Radio waves, Microwaves, infrared visible, ultraviolet, X-rays, gamma rays). including elementary facts about their uses.
Ray optics and optical instruments: Ray optics: Reflection of light by spherical mirrors, Mirror formula, refraction of light, lateral shift of a ray refraction through a glass slab, real and apparent depth, Advance Sunrise and delayed sun set due to atmospheric refraction, Total internal reflection, and its applications, Optical fibre, Mirage, totally reflecting prism, Brilliancy of diamond, Refraction at convex spherical surfaces. Lenses, Refraction by a lens, Power of a lens, Combination of thin lenses and mirror in contact, lens formula lens maker’s formula. Refraction and Dispersion of light through a prism, pattering of light- blue colour of sky and reddish appear of the sun at sunrise and sunset. Some Natural phenomena due to sunlight- The rainbow.
Optical instruments: – Microscopes and Astronomical telescopes (reflecting and refracting) and their magnifying powers. Atoms: Alpha- particle Scattering experiment. Rutherford’s Model of atom, Drawbacks of Rutherford’s Model. Bohr’s Model of hydrogen atom- postulates, Bohr radius, Total Energy of electron in nth orbit. Energy levels of hydrogen atom The line spectra of hydrogen atom. Nuclei: Atomic masses & composition of Nucleus, Size of nucleus isotopes, isobars isotones Mass energy relation, Nuclear Binding Energy and Mass Defect. Binding Energy and Mass Defect. Binding Energy Curve, Radioactivity. Law of radioactive decay, Alpha, Beta & Gamma particles/rays and their properties, nuclear fission and fusion.
Solution: Types of solutions, expression of concentration of solution of solid in liquids, solubility of gases in liquids, (Henry’s law) solid solutions, colligative properties- relative lowering of vapour pressure, Raoults’s law, ideal and non-Ideal solutions, osmotic pressure, osmosis and it’s applications, depression of freezing point, elevation of boiling point, determination of molecular masses using colligative properties, Abnormal molecular masses, Van’t Hoff- factor, Van’t Hoff equation for colligative properties.
Electrochemistry: Redox reactions, conductance in electrolytic solutions, specific and molar conductivity, variation of conductivity with concentration, Kohlrausch’s law, electrolysis and laws of electrolysis (only elementary Idea), Electrochemical cell, (construction, representation and working) dry cell- electrolytic cells, lead accumulator, Ni-cd cell, fuel cell, EMF of a cell, standard electrode potential, Nernst equation and its application to chemical cells, reference electrode (NHE) Relation between Gibb’s free energy change and EMF of a cell, Electrochemical series and it’s applications.
Coordination compounds: Coordination compounds- Introduction, difference between Coordination compounds and double salts, ligands, coordination number, colour, magnetic properties and shapes, IUPAC nomenclature of mono nuclear Coordination compounds, bonding in complex compounds, Werner’s theory, VBT and CFT: Structure and stereo isomerism, importance of Coordination compounds (In qualitative inclusion, extraction of metals and biological system).
Structural Organization in Animals and Plant: Cell-The Unit of Life Cell theory and cell as the basic unit of life, structure of prokaryotic and eukaryotic cells; Plant cell and animal cell; cell envelope; cell membrane, cell wall; cell organelles – structure and function; endomembrane system, endoplasmic reticulum, Golgi bodies, lysosomes, vacuoles, mitochondria, ribosomes, plastids, microbodies; cytoskeleton, cilia, flagella, centrioles (ultrastructure and function); nucleus.
Biomolecules Chemical constituents of living cells: biomolecules, structure and function of proteins, carbohydrates, lipids, nucleic acids; Enzyme – types, properties, enzyme action. (Topics excluded: Nature of Bond Linking Monomers in a Polymer, Dynamic State of Body Constituents – Concept of Metabolism, Metabolic Basis of Living, The Living State) Cell Cycle and Cell Division: Cell cycle, mitosis, meiosis and their significance
Genetics and Evolution: Heredity and variation: Mendelian inheritance; deviations from Mentalism – incomplete dominance, co-dominance, multiple alleles and inheritance of blood groups, pleiotropy; elementary idea of polygenic inheritance; chromosome theory of inheritance; chromosomes and genes; Sex determination – in humans, birds and honey bee; linkage and crossing over; sex linked inheritance – haemophilia, colour blindness; Mendelian disorders in humans – thalassemia; chromosomal disorders in humans; Down’s syndrome, Turner’s and Klinefelter’s syndromes.
Molecular Basis of Inheritance: Search for genetic material and DNA as genetic material; Structure of DNA and RNA; DNA packaging; DNA replication; Central Dogma; transcription, genetic code, translation; gene expression and regulation – lac operon; Genome, Human and rice genome projects; DNA fingerprinting. Evolution: Origin of life; biological evolution and evidences for biological evolution (palaeontology, comparative anatomy, embryology and molecular evidences); Darwin’s contribution, modern synthetic theory of evolution; mechanism of evolution – variation (mutation and recombination) and natural selection with examples, types of natural selection; Gene flow and genetic drift; Hardy – Weinberg’s principle; adaptive radiation; human evolution.
Ecology and Environment: Organisms and Populations: Population interactions – mutualism, competition, predation, parasitism; population attributes – growth, birth rate and death rate, age distribution. (Topics excluded: Organism and its Environment, Major Abiotic Factors, Responses to Abiotic Factors, Adaptations) Ecosystems: Patterns, components; productivity and decomposition; energy flow; pyramids of number, biomass, energy (Topics excluded: Ecological Succession and Nutrient Cycles)
Biodiversity and its Conservation: Biodiversity-Concept, patterns, importance; loss of biodiversity; biodiversity conservation; hotspots, endangered organisms, extinction, Red Data Book, Sacred Groves, biosphere reserves, national parks, wildlife, sanctuaries and Ramsar sites.
Introduction of Bones of the Human Body of: Upper Limb: clavicle, scapula, humerus, radius, ulna, carpus, metacarpus & phalanges Lower Limb: hipbone, femur, tibia, fibula, tarsus, metatarsus & phalanges Skull: name the bone of skull and sutures between them
Thorax: ribs and their articulations Vertebral Column: Cervical, thoracic, lumber, sacral and cocasial vertebrae. Surface Markings of the Body: Nine regions of the abdomen
Four quadrants of the Hip Respiratory Organs: Nasopharynx Oropharynx Larynx Trachea Bronchi Lungs (and their lobular segments) Thoracic cavity Pleura and Pleural cavity Circulatory Organs: Anatomical position of the heart Pericardium of the heart Chambers of the heart Great vessels of the heart Valves of the heart Digestive Organs: Tongue Teeth Oral cavity Pharynx Oesophagus Stomach Small intestine Large intestine and its colons.
Reproductive Organs: Male & Female Conads: Testes, Epididymis, Ovary, Fallopian Tube, Uterus, Vagine etc., Introduction of male Genital Organs, Introduction of female Genital Organs. Liver and Spleen: Introduction, Anatomical Position,Gall bladder. Excretory Organs; Cortex and medulla of kidney, Ureter, Urinary Bladder, Urethra (male and female) Muscles: Introduction, Origin and Insertion, Function.
The Cell in health and disease: Introduction of pathology Cellular structure and metabolism Inflammation – Acute and Chronic Derangement of Body Fluids and Electrolytes, Types of shocks, Ischaemia, Infection Neoplasia – Etiology and Pathogenesis.
Introduction of haematology: Formation of Blood Erythropoiesis Leucopoiesis Thrombopoiesis Collection of Blood Anticoagulants Red cell count – Haemocytometer, Methods and Calculation WBC Count — Methods Differential Leucocytes Count (DLC)– Morphology of White Cells, Normal Values, Staining procedures Counting Methods, Principle of staining Hb estimation – Method Colorimetric, Method Chemical, Method Gas metric, Method S.G., Method Clinical Importance.
Haematology: ESR Methods Factors – Affecting ESR Normal Values Importance RBC – Indices WBC Platelets.
Body Fluids: Urine: Method of Collection Normal Constituents, Physical Examination, Chemical Examination, Stool Examination: Method of Collection Normal Constituents and appearance,Abnormal Constituents (Ova, Cyst), C.S.F. Examination, Physical Examination, Chemical Examination Microscopy, Cell 1 Count, Staining, Semen Analysis, Collection Examination Special Tests.
Human blood group: antigens and antibodies, ABO Blood group systems, Sub. – group, Source of antigens and types of antibodies, Rh Blood group System, Types of Antigens, Mode of Inheritance, Types of Antibodies, Other Blood group Antigens, Blood Collection, Selection and screening of donor, Collection of blood, Various anticoagulants,Storage of Blood, Changes in Blood on Storage.
Introduction of Biochemistry: Elementary knowledge of inorganic chemistry: – Atomic weight, molecular weight, equivalent weight, acid, bases. Definition and preparation of solutions: Percent solution, Molar solution, Normal Solution and Buffer Solution etc. Definition and preparation of Regent. Unit of measurement Elementary knowledge of organic chemistry Organic compounds Aliphatic and Aromatic Alcohols, Aldehydes, Ketones, Amines, Esters, Phenol etc Ph indicators: pH paper, universal and other indicators, pH measurement: different methods.
Glassware: Composition of Glass General Glass wares Instruments: Balance Hot Plate and Magnetic stirrer Centrifuges Incubators Constant temperature bath Colorimeter: Principal, Function Photometer Flame Photometry Aim and Scope of Biochemistry Collection and Recording of Biochemical Specimen, separation of serum/plasma preservation and disposal of biologicalmaterial. Chemical examination of urine: Qualitative, Sugar, Protein, Bile Salt, Bile Pigment,
Ketones Bodies Chemical examination of Stool: Occult Blood. Chemical examination of other Body Fluids: CSF, Plural Fluids, Ascitic Fluid etc. Laboratory management and Maintenance of Records. Urine Examination physical, Chemical, Microscopic, Biochemistry Stool Examination Body Fluids: Physical and chemical examination CSF, Pleural Fluids, and Ascitic fluid. Carbohydrates: – Introduction, Importance, Classification, Properties, Estimation of Glucose, Clinical Significance.
Protein: – Introduction and Physiological importance, Amino acids, Essential amino acids, Classification, Denaturation of Proteins, Estimation of Total protein, Albumin, Globulin, A/G Ratio Introduction, Properties and function of important hormones. Enzymes and Co-enzymes; Introduction and difference, Functions, Estimation of important enzymes, SGOT (AST), SGPT (ALT), Alkaline Phosphatase, Acid Phosphatase, Amylase, lactate dehydrogenase, CPK, CPK-MB. Lipids: – Introduction and functions, Classification Steroids, Metabolism Estimation: Total lipids, HDL, LDL, VLDL, Total cholesterol, Triglyceride, Clinical significance, Principal of Assay procedures for biological material and estimation of kidney function tests. Urea, Uric acid, Creatinine.
Electrolytes: Function Properties Estimation of Essential electrolytes: Sodium, potassium, calcium, chloride and Phosphorus etc. Clinical Importance Genetics DNA, RNA Structure Gene coding Transcription& Translation Genetic Disorders.
Introduction of brief history of Microbiology: Historical Aspect Relationship of Micro-organism to men, Micro-organism in Disease and Health, Requirement and uses of common Laboratory Equipment, Incubator, Hot Air Oven, Water Bath Anaerobic Jar, Centrifuge, Autoclave Microscope Glassware – Description of Glassware, its use, handling and care Sterilization: Definition Classification and General Principal of Sterilization Autoclave – its structure, functioning, control and indicator, Definition, Types Mode of Action Uses Collection, Transportation and processing of clinical samples for Microbiological Investigations.
Bacteriology: Definition Bacteria – General characteristics of Bacteria Classification and morphology of Bacteria Structure of Cell, Capsule, Flagella, and Spore Growth of Bacteria Nutrition of Bacteria. Virology: Definition General Introduction of Virus Physiochemical characteristic of Viruses Diseases caused by different Virus and mode of infection. Parasitology: Definition General Characteristics of Parasite Classification of Parasite Mode of transmission
Fungus: Definition Structure Classification
Staining of Bacteria: Composition and preparation of staining Principal and Procedure of Bacteriological stain Gram’s Stain Ziehl-Neelsen Stain Albert Stain Spore and Negative Stain.
Cultivation of Micro-organism: Introduction and uses of culture Classification of culture media Composition of common of Laboratory culture media Special media and preparations Techniques of inoculation and isolation antimicrobial sensitivity Anaerobic cultivation techniques. Isolation of Viruses in Laboratory by tissue culture: Cell and tissue culture technology, Embryonated Egg, Principles of animal cell culture and their use in Virology, Different staining techniques used in Virology, Principle of different serological test used in Virology, Mode of Transmission of Viral agents, Prevention of Viral disease Immunity in Viral infection.
Immunology: Definition,Immunity: Definition and Classification Antigen Antibodies – Immunoglobulin, Antigen and antibody, reaction and clinical importance, Structure and function of immune system, Immune response Hypersensitivity. Principal & Procedure of Serological Tests: BICAL, CRP, Brucella, Agglutination, ASO Cold agglutination, VDRL, TPHA Advanced techniques in Microbiology ELISA, RIA etc. Epidemiological Markers of Micro-organism serotyping Preparation & Standardization of Antigen and Antisera Preparation & Standardization of vaccine and immunization. General Introduction, life cycle, mode of transmission, pathogenicity, and lab diagnosis of various Protozoa: Entamoeba Hisolytica, Entamoeba coli, Giardia lamblia, Trichomonas Vaginalios, Leishmania Donovan.
Cestodes or Tapeworms: Taenia solium, Taenia sagnata,Hymenolepis nana,Echincoccusgranulosus. Trematodes of Flukes: Fasciola hepatica, Fasciola gigantea,Gestrodiscoides hominis. Nematodes: Trichinella spiralis, Trichuris trichiura,Ancylostoma duodenale,Enterobjus vermicularis, Ascaris lumbricoide. The important physic-chemical laws applied to physiology: Diffusion, Osmosis, Bonding, Filtration, Dialysis, Surface Tension, Adsorption Colloid. Fundamentals of different Organ Systems: Cardiovascular System, Respiratory System, Digestive System, Excretory System, Reproduction System, Endocrine System, Lymphatic System. Blood: Definition, Composition, Function, Formation of different type of blood cells; Erythrocytes, Leucocytes, Thrombocytes, Mechanism of Blood Clotting; Cerebrospinal Fluid Formation, Composition, Function, Special Senses; Hearing, Taste, Smell, Touch, Sight
How to Prepare for the HSSC Lab Technician Syllabus Exam
Start with the subject-specific block of the HSSC Lab Technician Syllabus first — it carries the heaviest weightage and is where real differentiation between candidates happens, since general awareness and computer knowledge sections overlap with every other HSSC group. Work through each topic area systematically, and revisit the subject list above every week so nothing slips through before exam day.
Don’t skip the common portion either. Haryana-specific general awareness and basic computer knowledge are easy marks that candidates often under-prepare because they’re focused on the technical syllabus.
Frequently Asked Questions: HSSC Lab Technician Syllabus
What is the HSSC Lab Technician Syllabus for Group 49?
The HSSC Lab Technician Syllabus covers the weighted sections listed above, compiled directly from the official document published by the Haryana Staff Selection Commission.
Is the HSSC Lab Technician Syllabus the same every year?
HSSC updates its syllabus documents periodically, and the weightage listed against each section is explicitly marked tentative by HSSC. Always check hssc.gov.in/examsyllabus for the latest version before you finalize your preparation.
Do I need to clear CET before attempting this exam?
Yes. HSSC recruitment follows the standard two-stage process: you first clear the Common Eligibility Test (CET), then sit for the group-specific written exam that follows the HSSC Lab Technician Syllabus covered in this guide.
Official Notification and Source
This HSSC Lab Technician Syllabus is compiled directly from the official Group 49 – Lab Technician Syllabus published by the Haryana Staff Selection Commission. Always cross-check the latest version and any amendments against the official document before you finalize your preparation.
Download the official syllabus PDF and check current recruitment notices at hssc.gov.in/examsyllabus.
Explore Other HSSC Group Syllabus Guides
This HSSC Lab Technician Syllabus guide is part of a growing set of subject-wise syllabus posts for every HSSC group. Browse the full set on the HSSC Syllabus tag page or the Syllabus category. For the full HSSC recruitment process, CET details and exam pattern, see the Haryana HSSC guide.